Chromatin Immunoprecipitation:Article Title: TLR sorting by Rab11 endosomes maintains intestinal epithelial-microbial homeostasis
Article Snippet: The sources of antibodies, proteins, and inhibitors are as follows: mouse anti-Rab11a (BD Transduction Laboratories, #610656), rabbit anti-Rab11a (US Biological, R0009), rat anti-BrdU (Accurate Chemicals, OBT0030G), mouse anti-E-Cadherin (BD Transduction Laboratories, #610181), rabbit anti-phosphorylated histone H3 (Millipore, MABE13), rat anti-BrdU (AbD Serotec, OBT0030G), goat anti-GFP (Abcam, ab6673), rabbit anti-chromogranin A (Immunostar, #20085), rabbit anti-Lyz (Biogenex, AR0245R), alkaline phosphatase kit (Vector Laboratories, SK-5100), rat anti-F4/80 (AbD Serotec, MCA497GA), goat anti-IL6 (R&D systems, AF-406-NA), mouse IL6 (Cell Signaling, #6514), rabbit anti-phosphorylated p38 MAPK (Cell Signaling, #4511), rabbit anti-p38 MAPK (Cell Signaling, #8690), rabbit anti-phosphorylated JNK (Cell Signaling, #4668), rabbit anti-JNK (Cell Signaling, #9258), rabbit anti-phosphorylated Stat3 (Cell Signaling, #9145), mouse anti-Stat3 (Cell Signaling, #9139), rabbit anti-P65 (Cell Signaling, #8242), rabbit anti-RelB (Cell Signaling, #4922), rabbit anti-Villin (Santa Cruz, sc-7672), rabbit anti-lamin A/C (Santa Cruz, sc-20681), mouse anti-β-Actin (Santa Cruz, sc-8432), rabbit anti-β-Catenin (Abcam, ab32572), mouse anti-TLR9 (Imgenex, IMG-305A), rabbit anti-TLR9 (Cell Signaling, #5845), rabbit anti-Rab7 (Cell Signaling, #9367), rabbit anti-MyD88 (Cell Signaling, #4283), rabbit anti-phosphorylated IKKα/β (Cell Signaling, #2697), rabbit anti-phosphorylated ERK (Cell Signaling, #3470), antiLAMP2 (Developmental Studies Hybridoma Bank, ABL-93), TO-PRO-3 (Invitrogen, T3605), BAY11-0782 (Santa Cruz, sc-200615).
Article Title: TASL is the SLC15A4-associated adaptor for IRF5 activation by TLR7-9.
Article Snippet: Antibodies used in immunofluorescence or flow cytometry Rabbit anti-HA (Cell Signaling, Cat.#: 3724, Lot #: 9, Dilution: 1:400), mouse anti-Lamp1 (Abcam, Cat.#: ab25630, Lot #: 792614, Dilution 1:200), Alexa Fluor 488-coupled goat anti-mouse (Thermo Fisher Scientific, Cat.#: A11001, Lot#: 2015565, Dilution: 1:400), Alexa Fluor 594-coupled goat anti-rabbit (Thermo Fisher Scientific, Cat.#: A11012, Lot#: 1892265, Dilution: 1:400), mouse anti-PD-L1-APC (Thermo Fisher Scientific, Cat.#: 17-5983-42, Lot #:4347834, Dilution: 1:20).
Flow Cytometry:Article Title: TLR sorting by Rab11 endosomes maintains intestinal epithelial-microbial homeostasis
Article Snippet: The sources of antibodies, proteins, and inhibitors are as follows: mouse anti-Rab11a (BD Transduction Laboratories, #610656), rabbit anti-Rab11a (US Biological, R0009), rat anti-BrdU (Accurate Chemicals, OBT0030G), mouse anti-E-Cadherin (BD Transduction Laboratories, #610181), rabbit anti-phosphorylated histone H3 (Millipore, MABE13), rat anti-BrdU (AbD Serotec, OBT0030G), goat anti-GFP (Abcam, ab6673), rabbit anti-chromogranin A (Immunostar, #20085), rabbit anti-Lyz (Biogenex, AR0245R), alkaline phosphatase kit (Vector Laboratories, SK-5100), rat anti-F4/80 (AbD Serotec, MCA497GA), goat anti-IL6 (R&D systems, AF-406-NA), mouse IL6 (Cell Signaling, #6514), rabbit anti-phosphorylated p38 MAPK (Cell Signaling, #4511), rabbit anti-p38 MAPK (Cell Signaling, #8690), rabbit anti-phosphorylated JNK (Cell Signaling, #4668), rabbit anti-JNK (Cell Signaling, #9258), rabbit anti-phosphorylated Stat3 (Cell Signaling, #9145), mouse anti-Stat3 (Cell Signaling, #9139), rabbit anti-P65 (Cell Signaling, #8242), rabbit anti-RelB (Cell Signaling, #4922), rabbit anti-Villin (Santa Cruz, sc-7672), rabbit anti-lamin A/C (Santa Cruz, sc-20681), mouse anti-β-Actin (Santa Cruz, sc-8432), rabbit anti-β-Catenin (Abcam, ab32572), mouse anti-TLR9 (Imgenex, IMG-305A), rabbit anti-TLR9 (Cell Signaling, #5845), rabbit anti-Rab7 (Cell Signaling, #9367), rabbit anti-MyD88 (Cell Signaling, #4283), rabbit anti-phosphorylated IKKα/β (Cell Signaling, #2697), rabbit anti-phosphorylated ERK (Cell Signaling, #3470), antiLAMP2 (Developmental Studies Hybridoma Bank, ABL-93), TO-PRO-3 (Invitrogen, T3605), BAY11-0782 (Santa Cruz, sc-200615).
Article Title: TASL is the SLC15A4-associated adaptor for IRF5 activation by TLR7-9.
Article Snippet: Antibodies used in immunofluorescence or flow cytometry Rabbit anti-HA (Cell Signaling, Cat.#: 3724, Lot #: 9, Dilution: 1:400), mouse anti-Lamp1 (Abcam, Cat.#: ab25630, Lot #: 792614, Dilution 1:200), Alexa Fluor 488-coupled goat anti-mouse (Thermo Fisher Scientific, Cat.#: A11001, Lot#: 2015565, Dilution: 1:400), Alexa Fluor 594-coupled goat anti-rabbit (Thermo Fisher Scientific, Cat.#: A11012, Lot#: 1892265, Dilution: 1:400), mouse anti-PD-L1-APC (Thermo Fisher Scientific, Cat.#: 17-5983-42, Lot #:4347834, Dilution: 1:20).
Magnetic Resonance Imaging:Article Title: TLR sorting by Rab11 endosomes maintains intestinal epithelial-microbial homeostasis
Article Snippet: The sources of antibodies, proteins, and inhibitors are as follows: mouse anti-Rab11a (BD Transduction Laboratories, #610656), rabbit anti-Rab11a (US Biological, R0009), rat anti-BrdU (Accurate Chemicals, OBT0030G), mouse anti-E-Cadherin (BD Transduction Laboratories, #610181), rabbit anti-phosphorylated histone H3 (Millipore, MABE13), rat anti-BrdU (AbD Serotec, OBT0030G), goat anti-GFP (Abcam, ab6673), rabbit anti-chromogranin A (Immunostar, #20085), rabbit anti-Lyz (Biogenex, AR0245R), alkaline phosphatase kit (Vector Laboratories, SK-5100), rat anti-F4/80 (AbD Serotec, MCA497GA), goat anti-IL6 (R&D systems, AF-406-NA), mouse IL6 (Cell Signaling, #6514), rabbit anti-phosphorylated p38 MAPK (Cell Signaling, #4511), rabbit anti-p38 MAPK (Cell Signaling, #8690), rabbit anti-phosphorylated JNK (Cell Signaling, #4668), rabbit anti-JNK (Cell Signaling, #9258), rabbit anti-phosphorylated Stat3 (Cell Signaling, #9145), mouse anti-Stat3 (Cell Signaling, #9139), rabbit anti-P65 (Cell Signaling, #8242), rabbit anti-RelB (Cell Signaling, #4922), rabbit anti-Villin (Santa Cruz, sc-7672), rabbit anti-lamin A/C (Santa Cruz, sc-20681), mouse anti-β-Actin (Santa Cruz, sc-8432), rabbit anti-β-Catenin (Abcam, ab32572), mouse anti-TLR9 (Imgenex, IMG-305A), rabbit anti-TLR9 (Cell Signaling, #5845), rabbit anti-Rab7 (Cell Signaling, #9367), rabbit anti-MyD88 (Cell Signaling, #4283), rabbit anti-phosphorylated IKKα/β (Cell Signaling, #2697), rabbit anti-phosphorylated ERK (Cell Signaling, #3470), antiLAMP2 (Developmental Studies Hybridoma Bank, ABL-93), TO-PRO-3 (Invitrogen, T3605), BAY11-0782 (Santa Cruz, sc-200615).
Article Title: TASL is the SLC15A4-associated adaptor for IRF5 activation by TLR7-9.
Article Snippet: Antibodies used in immunofluorescence or flow cytometry Rabbit anti-HA (Cell Signaling, Cat.#: 3724, Lot #: 9, Dilution: 1:400), mouse anti-Lamp1 (Abcam, Cat.#: ab25630, Lot #: 792614, Dilution 1:200), Alexa Fluor 488-coupled goat anti-mouse (Thermo Fisher Scientific, Cat.#: A11001, Lot#: 2015565, Dilution: 1:400), Alexa Fluor 594-coupled goat anti-rabbit (Thermo Fisher Scientific, Cat.#: A11012, Lot#: 1892265, Dilution: 1:400), mouse anti-PD-L1-APC (Thermo Fisher Scientific, Cat.#: 17-5983-42, Lot #:4347834, Dilution: 1:20).
Western Blot:Article Title: TLR sorting by Rab11 endosomes maintains intestinal epithelial-microbial homeostasis
Article Snippet: The sources of antibodies, proteins, and inhibitors are as follows: mouse anti-Rab11a (BD Transduction Laboratories, #610656), rabbit anti-Rab11a (US Biological, R0009), rat anti-BrdU (Accurate Chemicals, OBT0030G), mouse anti-E-Cadherin (BD Transduction Laboratories, #610181), rabbit anti-phosphorylated histone H3 (Millipore, MABE13), rat anti-BrdU (AbD Serotec, OBT0030G), goat anti-GFP (Abcam, ab6673), rabbit anti-chromogranin A (Immunostar, #20085), rabbit anti-Lyz (Biogenex, AR0245R), alkaline phosphatase kit (Vector Laboratories, SK-5100), rat anti-F4/80 (AbD Serotec, MCA497GA), goat anti-IL6 (R&D systems, AF-406-NA), mouse IL6 (Cell Signaling, #6514), rabbit anti-phosphorylated p38 MAPK (Cell Signaling, #4511), rabbit anti-p38 MAPK (Cell Signaling, #8690), rabbit anti-phosphorylated JNK (Cell Signaling, #4668), rabbit anti-JNK (Cell Signaling, #9258), rabbit anti-phosphorylated Stat3 (Cell Signaling, #9145), mouse anti-Stat3 (Cell Signaling, #9139), rabbit anti-P65 (Cell Signaling, #8242), rabbit anti-RelB (Cell Signaling, #4922), rabbit anti-Villin (Santa Cruz, sc-7672), rabbit anti-lamin A/C (Santa Cruz, sc-20681), mouse anti-β-Actin (Santa Cruz, sc-8432), rabbit anti-β-Catenin (Abcam, ab32572), mouse anti-TLR9 (Imgenex, IMG-305A), rabbit anti-TLR9 (Cell Signaling, #5845), rabbit anti-Rab7 (Cell Signaling, #9367), rabbit anti-MyD88 (Cell Signaling, #4283), rabbit anti-phosphorylated IKKα/β (Cell Signaling, #2697), rabbit anti-phosphorylated ERK (Cell Signaling, #3470), antiLAMP2 (Developmental Studies Hybridoma Bank, ABL-93), TO-PRO-3 (Invitrogen, T3605), BAY11-0782 (Santa Cruz, sc-200615).
Article Title: TASL is the SLC15A4-associated adaptor for IRF5 activation by TLR7-9.
Article Snippet: Antibodies used in immunofluorescence or flow cytometry Rabbit anti-HA (Cell Signaling, Cat.#: 3724, Lot #: 9, Dilution: 1:400), mouse anti-Lamp1 (Abcam, Cat.#: ab25630, Lot #: 792614, Dilution 1:200), Alexa Fluor 488-coupled goat anti-mouse (Thermo Fisher Scientific, Cat.#: A11001, Lot#: 2015565, Dilution: 1:400), Alexa Fluor 594-coupled goat anti-rabbit (Thermo Fisher Scientific, Cat.#: A11012, Lot#: 1892265, Dilution: 1:400), mouse anti-PD-L1-APC (Thermo Fisher Scientific, Cat.#: 17-5983-42, Lot #:4347834, Dilution: 1:20).
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